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Application of seed germination pouch for culture and initial resistance screening of the soybean cyst nematode Heterodera glycines
J. You, Y. F. Hu and C. L. Wang
2018
发表期刊Nematology
卷号20页码:905-909
摘要Seed germination pouches or seedling growth pouches have been utilised previously for high throughput resistance screening of root-knot nematodes, Meloidogyne spp., on legume plants. In this study we demonstrated that soybean cyst nematode (SCN) Heterodera glycines, with a different reproduction mode from root-knot nematode, is also able to invade, complete its life cycle and reproduce in soybean roots in seedling pouches similar to the pot system with soil, indicating seedling pouches can be utilised as an alternate SCN culture method when glasshouse/growth chamber space is limited. Moreover, seedling pouches provide an easy approach to collect large numbers of clean male nematodes, which could accelerate SCN biological study such as SCN mating behaviour and molecular signals associated with mating. The results of SCN resistance screening indicate that seedling pouches could be utilised for initial resistance screening with SCN.
文献类型期刊论文
条目标识符http://ir.iga.ac.cn/handle/131322/9115
专题中国科学院东北地理与农业生态研究所
作者单位中国科学院东北地理与农业生态研究所
推荐引用方式
GB/T 7714
J. You, Y. F. Hu and C. L. Wang. Application of seed germination pouch for culture and initial resistance screening of the soybean cyst nematode Heterodera glycines[J]. Nematology,2018,20:905-909.
APA J. You, Y. F. Hu and C. L. Wang.(2018).Application of seed germination pouch for culture and initial resistance screening of the soybean cyst nematode Heterodera glycines.Nematology,20,905-909.
MLA J. You, Y. F. Hu and C. L. Wang."Application of seed germination pouch for culture and initial resistance screening of the soybean cyst nematode Heterodera glycines".Nematology 20(2018):905-909.
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